Sunday, July 12, 2009

Tomography

About a month ago I posted an entry about the microscopes in my lab. In this previous post, I briefly described a technique called Electron Tomography. Here is great video that describes in detail the process of making a tomogram. The section used for this was 500 nanometers thick. This is extremely thick for an electron microscope. The data taken for this tomogram was gathered on one of the world's three 1 Million kV transmission electron microscopes! Most microscopes only have the capability to gather images on sections no thicker than 200 nanometers.

About a year ago I was in a class at the New York Structural Biology Center about electron microscopy techniques. The lesson about electron tomography was taught by the person who made this particular tomogram.

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