Tuesday, June 9, 2009

Microscopes in the lab

Here are a couple of pictures I took of the two Transmission Electron Microscopes (click to enlarge) that are currently in our lab. I Photoshoped them a little to cut out the background. On the left is the Philips CM-12. This is our work-horse and the instrument that I use the most. It's source is a Tungsten Filament that can run at 120kV. Most of the TEM images that you will see on this blog were taken using this microscope.
On the right is the Philips CM-200. This microscope is mostly used by the structural biologists in the institute for studying protein crystals and single particles. It's source is a Field Emission Gun that can run at 200kV. The CM-200 has a special cryo-stage for doing cryo-electron microscopy can also be controlled by a computer. This is handy for doing Electron Tomography, a special type of imaging that can make 3D reconstructions. See movie below.

By the way, I can't take credit for this video. Whoever created it likely spent years developing getting the right conditions for the sample and many many hours on image acquisition and processing.

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