This is a 500 nm thick cross section of the eye of a guppy embryo. The lens (bottom part) of the eye was a bit deformed, but I think the rest of it survived the sample processing in tact. It was stained with Polychrome Blue and imaged on an optical light microscope. The interesting thing about this image is how it was constructed. I used a 40x lens and captured about 40 images. They were then manually stitched together in Photoshop (I was using CS1, when there was no option to do it automatically). Once completed, the image was 14,400 x 10,800 pixels (about 155 megapixels) and storage about a half of a gigabyte! I drastically reduced the size in order to publish it here, but I think it still looks pretty crisp. The original version could be zoomed in and viewed at high magnification.
Showing posts with label guppy. Show all posts
Showing posts with label guppy. Show all posts
Tuesday, June 9, 2009
Guppy Eye
If you couldn't tell by now, I did a big project with guppy embryos when I was in school. Here's another image. Click to enlarge.
This is a 500 nm thick cross section of the eye of a guppy embryo. The lens (bottom part) of the eye was a bit deformed, but I think the rest of it survived the sample processing in tact. It was stained with Polychrome Blue and imaged on an optical light microscope. The interesting thing about this image is how it was constructed. I used a 40x lens and captured about 40 images. They were then manually stitched together in Photoshop (I was using CS1, when there was no option to do it automatically). Once completed, the image was 14,400 x 10,800 pixels (about 155 megapixels) and storage about a half of a gigabyte! I drastically reduced the size in order to publish it here, but I think it still looks pretty crisp. The original version could be zoomed in and viewed at high magnification.
This is a 500 nm thick cross section of the eye of a guppy embryo. The lens (bottom part) of the eye was a bit deformed, but I think the rest of it survived the sample processing in tact. It was stained with Polychrome Blue and imaged on an optical light microscope. The interesting thing about this image is how it was constructed. I used a 40x lens and captured about 40 images. They were then manually stitched together in Photoshop (I was using CS1, when there was no option to do it automatically). Once completed, the image was 14,400 x 10,800 pixels (about 155 megapixels) and storage about a half of a gigabyte! I drastically reduced the size in order to publish it here, but I think it still looks pretty crisp. The original version could be zoomed in and viewed at high magnification.
Monday, June 8, 2009
D'na..d'na, d'na, d'na, d'na..wrEEEEEEEE! *jaws theme*
Did you know that guppies have teeth? I didn't 'till i put it in the SEM. This is another one from the guppy embryos back in school.
These images were taken on a Hitachi2700 SEM.
These images were taken on a Hitachi2700 SEM.
Thursday, June 4, 2009
A few pics
Let's add some fun pics. I've been in the EM field for a few years now. Here are a few images from back in school. All can be clicked to view them larger. More current work will follow.
This is a diatome. In the background are broken up ones. This was gathered from some diatomaceuos earth that we bought to keep slugs away from the veggies we were growing. The image was gathered with a Hitachi 2700 SEM and colorized in Photoshop.
This is some moss I found growing between two pieces of sidewalk. The image was gathered with a Hitachi 2700 SEM and colorized in Photoshop.
Here are a few images of some gills from a guppy embryo. Some were freeze fractured with liquid nitrogen to expose the contents. The images were gathered on a JEOL 840 SEM. One was colorized in Photoshop.
This is a diatome. In the background are broken up ones. This was gathered from some diatomaceuos earth that we bought to keep slugs away from the veggies we were growing. The image was gathered with a Hitachi 2700 SEM and colorized in Photoshop.
This is some moss I found growing between two pieces of sidewalk. The image was gathered with a Hitachi 2700 SEM and colorized in Photoshop.
Here are a few images of some gills from a guppy embryo. Some were freeze fractured with liquid nitrogen to expose the contents. The images were gathered on a JEOL 840 SEM. One was colorized in Photoshop.
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